Dehydration, Rehydration, and Impact on Physiology

Part of paid clinical trials in Lubbock, Texas.

Sponsor
Texas Tech University
Study ID
NCT07084675
Status
Recruiting
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Conditions

  • Aging

Eligibility Criteria

Sex
ALL
Age
45 Years - 65 Years
Healthy Volunteers
Accepted

Interventions

  • Hydration-Dehydration-Rehydration Protocol (HDR) — BEHAVIORAL
    The HDR Protocol is a 12-day, sequential fluid manipulation intervention designed to assess the physiological effects of controlled changes in hydration status. The protocol consists of three consecutive 4-day phases: Hydration Phase (Days 1-4): Participants follow a standardized fluid intake protocol designed to promote and maintain a euhydrated state. Hydration status is monitored using objective measures such as urine specific gravity and body mass. Dehydration Phase (Days 5-8): Participants adhere to a fluid-restriction regimen to induce a hypohydrated state. The level of dehydration is verified through the same hydration status measures to ensure consistent and safe hypohydration. Rehydration Phase (Days 9-12): Participants resume the standardized fluid intake protocol used during the Hydration Phase to restore euhydration. Rehydration effectiveness is confirmed through repeated hydration assessments.

Study Details

Our goal is to determine whether hydration status influences inflammation, and overall physiological performance in adults aged 45-65. Aim 1: The effects of 4-day mild dehydration followed by 4-day rehydration (defined as approximately 2-3% body mass loss) on H2O2, upstream inflammatory signal (NFkB), mitochondrial fragmentation, and autophagy in circulating PBMCs. Aim 2: The effects of 4-day mild dehydration followed by 4-day rehydration (defined as approximately 2-3% body mass loss) on fasting glucose, sleep performance, and strength performance. Participant will: complete a 4-day hydration protocol, followed by a 4-day dehydration protocol, and then a 4-day rehydration protocol. attend five laboratory visits for testing: Day 1: Informed consent and familiarization Day 2: Pre-hydration testing (before the 4-day hydration protocol) Day 3: Pre-dehydration testing (before the dehyration protocol) Day 4: Post-dehydration testing (after the dehydration protocol) Day 5: Post-rehydration testing (after the rehydration protocol)

Key Dates

Start date
Jul 15, 2025
Status verified
Sep 2025
Primary completion
Dec 31, 2025
Completion
Dec 31, 2026

Study Design

Enrollment
15 participants (estimated)
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
PREVENTION

Arms

  • Experimental: Hydration-Dehydration-Rehydration (HDR) Protocol Arm
    Participants in this arm complete the HDR Protocol over 12 days and attend five lab visits.

Primary Outcome Measure

Fasting blood glucose (mg/dL) [ Time Frame: Day 2 (Pre-hydration testing), Day 3 (Pre-dehydration testing), Day 4 (Post-dehydration testing), Day 5 (Post-rehydration testing) ]

Central Contacts

Locations (1)

FacilityCityStateZIPSite coordinators
Texas Tech UniversityLubbockTexas79409
Hui Ying Luk, Ph.D.
[email protected]
806-834-0827

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By research site
Texas Tech University· Lubbock, TX

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