Measuring Protein Turnover in Humans Across the Lifespan by Metabolic Labeling With Deuterium Oxide

Part of paid clinical trials in Baltimore, Maryland.

Sponsor
National Institute on Aging (NIA)
Study ID
NCT06269653
Status
Recruiting
Apply to this trial

Conditions

  • Healthy Volunteers

Eligibility Criteria

Sex
ALL
Age
20 Years - 120 Years
Healthy Volunteers
Accepted

Study Details

Background: Proteins are essential to the health and structure of the cells that make up body tissues. Most proteins become damaged over time and are replaced with new ones. This process is called "protein turnover." Stress, disease, and aging can affect this process. Researchers want to better understand how aging affects protein turnover. Objective: To measure rates of protein turnover in healthy adults. Eligibility: Healthy people aged 20 years and older with a body mass index between 20 and 30. Design: Participants will have 6 study visits over 4 to 6 weeks. They will fast 12 hours before each visit. Participants will be screened. They will have a physical exam, with blood and urine tests and tests of their heart function. They will lie down while blood pressure cuffs are used on their arms and legs. Participants will be given bottles of heavy water to drink at home on a schedule for 21 days. Each bottle holds about 3.5 tablespoons. Heavy water is odorless, colorless, and tasteless, like normal drinking water. It is safe to drink and has been used in research for many years. Participants will have tests during study visits, including: Imaging scans of a leg. Exercise on a treadmill. Biopsies of muscle, skin, and fat: Small samples of tissue will be cut from the calf and abdomen. Resting metabolic rate: Participants will lie still and breathe into a mask for 20 minutes. Knee/grip strength: Participants will do strength tests with their legs. They will squeeze a device with their hands. D3-Creatine: Participants will take 1 pill of D3-Creatine, which occurs naturally in muscle.

Key Dates

Start date
Jun 26, 2024
Status verified
Feb 2026
Primary completion
Mar 1, 2027
Completion
Mar 1, 2027

Study Design

Enrollment
75 participants (estimated)

Arms

  • Arm: Healthy Individuals: 20 - 29 years of age
    Age group: 20 -29 (3 men and 3 women)
  • Arm: Healthy Individuals: 30 - 39 year of age
    Age group: 30 - 39 (3 men and 3 women)
  • Arm: Healthy Individuals: 40 - 49 years of age
    Age group 40 - 49 (3 men and 3 women)
  • Arm: Healthy Individuals: 50 - 59 years of age
    Age group: 50 - 59 (3 men and 3 women)
  • Arm: Healthy Individuals: 60 - 69 years of age
    Age group: 60 - 69 (3 men and 3 women)
  • Arm: Healthy Individuals: 70 +
    Age group: 70+ (3 men and 3 women)

Primary Outcome Measure

Measure the turnover proteins in humans across tissues, age, and sex. [ Time Frame: 21 days ]

Central Contacts

Locations (1)

FacilityCityStateZIPSite coordinators
National Institute of Aging, Clinical Research UnitBaltimoreMaryland21224
Luigi Ferrucci, M.D.
[email protected]
410-558-8110

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By research site
National Institute of Aging, Clinical Research Unit· Baltimore, MD

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