In Vivo Assessment of Glutamine Utilization by Bone Marrow Plasma Cells

Part of paid clinical trials in Rochester, Minnesota.

Sponsor
Mayo Clinic
Study ID
NCT03384108
Status
Recruiting
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Conditions

  • Healthy

Eligibility Criteria

Sex
ALL
Age
18 Years - 60 Years
Healthy Volunteers
Accepted

Interventions

  • 5-13C-Glutamine — OTHER
    Intravenous infusion of 5-13C-Glutamine
  • Bone marrow aspiration — OTHER
    Bone marrow aspiration of bone marrow aspirate

Study Details

This pilot study will first establish the feasibility of an in vivo methodology of assessing the utilization of glutamine into the TCA cycle of normal bone marrow plasma cells from healthy subjects while comparing it to an ex vivo approach

Key Dates

Start date
Sep 1, 2017
Status verified
Feb 2026
Primary completion
Jan 31, 2028
Completion
Jan 31, 2028

Study Design

Enrollment
20 participants (estimated)
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
DIAGNOSTIC

Arms

  • Experimental: In Vivo
    Infusion of 5-13C-Glutamine intravenously in healthy subjects prior to undergoing a bone marrow aspiration.
  • Placebo Comparator: Ex Vivo
    Healthy subjects will undergoing a bone marrow aspiration and then the plasma cells acquired will be cultured ex vivo in cell culture media containing 5-13C-Glutamine.

Primary Outcome Measure

Percentage of enrichment of 13-Carbon molecules in the TCA cycle metabolites of plasma cells measured by gas chromatography mass spectrometry. [ Time Frame: Through study completion, an average of 1 hour ]

Central Contacts

Locations (1)

FacilityCityStateZIPSite coordinators
Mayo ClinicRochesterMinnesota55905
Wilson Gonsalves, MD
[email protected]
5072842511

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Mayo Clinic· Rochester, MN

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