Molecular Analysis Of Solid Tumors

Conditions

• Pediatric Solid Tumors

Eligibility Criteria

Sex

ALL

Age

N/A - 25 Years

Healthy Volunteers

Not accepted

Study Details

This study will prospectively characterize the molecular, cellular and genetic properties of primary and metastatic neuroblastoma, osteosarcoma, retinoblastoma, Ewing sarcoma family of tumors, soft tissue sarcomas, adrenocortical tumors and liver malignancies. These cell isolates will be used for gene expression array analysis, genomic analysis by \[SNP\] single nucleotide polymorphism chip, array \[CGH\] comparative genomic hybridization and next generation sequencing, and \[TEM\] transmission electron microscopy analysis. Additionally cell lines and orthotopic xenografts will be created from the obtained tumor specimens. The specificity of TCRs will be examined by comparing paired TCR from peripheral blood and tumor infiltrating CD4+ and CD8+ T cells. Epigenetic studies will be performed looking at the methylation profile of these cells and to investigate the anti-tumor T cell response both pre- and post-PD1 inhibition.

Key Dates

Start date

Feb 10, 2010

Status verified

Nov 2025

Primary completion

Sep 30, 2026

Completion

Sep 30, 2026

Study Design

Enrollment

1,000 participants (estimated)

Primary Outcome Measure

Perform analysis of gene expression profiles. [RNA] ribonucleic acid will be isolated from fresh frozen tumor specimens and hybridized to Affymetrix gene expression arrays. [ Time Frame: 5 years ]

Central Contacts

• Sara M. Federico, MD
  1-866-278-5833
  [email protected]

Locations (1)

Find similar trials in Memphis, TN

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